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Antibacterial and phytochemical screening of pimpinella anisum through optimized extraction procedure

Author: 
Zubaida Marufee Islam, Kashmery Khan, Shagoofa Rakhshanda, Rabab Mahdi and Iftekhar Mahmud Chowdhury
Abstract: 

Since synthetic drugs failed to work on microbial resistance we focused on antimicrobial properties of naturally occurring Pimpinella anisum, which may help in the search for newer and less expensive antibiotics. Antibacterial effects of ethanol and methanol extracts taken of second, fifth and seventh days, along with aqueous extracts of aniseed were observed on nine bacteria by disc diffusion method. Aniseed extracts of different days showed positive antibacterial effects on only three bacteria, named, Bacillus cereus, Bacillus subtilis and Streptococcus pneumonae. The methanol extract from the 5th day showed the highest positive result against B. cereus whereas the zone of inhibition for B. subtilis was maximum for the 7th day of ethanol extracts. Additionally, the aqueous extract of aniseed showed unremarkable result against S. pneumonae. Again, the activity index of methanol extract of day five was the highest against Bacillus subtilis indicating high sensitivity to the extract. The phytochemical analysis of the aqueous extract revealed the presence of alkaloids, flavonoids, saponins, tannins, terpenoids, phenolic compounds and cardiac glycosides. As extraction procedure is optimized through this examination, it is expected that the findings of this study will stimulate researchers to design clinical trials that may lead to the development of less expensive antimicrobial agents.

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