Call for Papers : Volume 15, Issue 03, March 2024, Open Access; Impact Factor; Peer Reviewed Journal; Fast Publication

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Exploration of sediment derived streptomyces sp. for the production of endoglucanase enzyme

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Among the cellulases, which break down the polymeric cellulose, are endoglucanase that acts synergistically for subsequent hydrolytic reactions. In the present investigation, sediment derived isolates of Actinomycetes were screened for endoglucanase enzyme and the potential isolate, Streptomyces sp. was used further for the isolation of enzyme. In microbial enzyme screening experiment, Streptomyces sp. showed the production of endoglucanase enzyme. The Endoglucanase activity was found to be 2.75U/ml. From among the three media used Vogel’s medium showed a higher activity for Endoglucanase with 1% rice straw (0.2U/ml). The enzyme was highly stable in a broad range of buffers for the test incubation period (1 h). More than 40% of initial activity was retained after 5 h incubation at pH 6.0. At acidic pH of 3 the enzyme showed about 86% of activity after which the activity was completely lost due to precipitation of enzyme. 10 – 15 % of concentrated Endoglucanase showed good results in liquefaction. These results highlight the importance of this isolate for the production of potential endoglucanase enzyme.

Author: 
Patil R.C. and Jadhav, B. L.
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