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Fed-batch and cstr studies for l-methionine production by c.glutamicum using agricultural Products

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Author: 
Venkata Narayana, A. and Swamy, A.V.N.
Abstract: 

The optimized values (nutrient and process parameters) obtained from RSM and ANN in the previous works were used to carry out fed-batch and CSTR studies for the production of L-Methionine by C.glutamicum using agricultural products as substrates. The optimized values obtained from earlier work were plantain as C-source (21.19 g/l), groundnut as N-source (11.20 g/l), MgSO4.7H2O (1.21 g/l) and inoculum size (3.30 ml). Determined the productivities (Dx and Dp) and yield coefficients (YX/S and YP/S) as functions of dissolved oxygen (DO) and dilution rate (D) in continuous fermentation of methionine. And also determined the yield coefficients and productivities for fed-batch culture and made comparison of results. The effect of dissolved oxygen between 5% and 50% and dilution rate between 0.05 and 0.3 was studied on continuous production of methionine using CCD based on RSM.

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