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A study on the heavy hydrocarbon degradation potential of bacterial strains isolated from Uttarakhand

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Author: 
Shweta Rawat
Abstract: 

Screening of hydrocarbon degrading microorganisms from crude oil contaminated soil (near Indian Institute of Petroleum, Dehradun) by selective enrichment technique, resulted in the collection of two distinct study species (Bacillus sp.and Micrococcus sp.). Both strains were firstly cultivated in liquid media with Glucose as the carbon and energy source. Morphological characteristics of strains were determined by preparing nutrient agar plates parallel. Further, both strains growth was examined by using four different hydrocarbons (n-Parrafin, Xylene and Naphthelenemixture (1:1), Petroleum coke and Lube aromatics) as a sole carbon and energy source in different set of experiments. Growth study and kinetics data of both strains in these set of experiments were studied and analysed. The results directly indicate the different hydrocarbon degradation potential of both strains.