The 180 patients of both gender between 10 80 years having symptoms of dyspepsia, are subjected to endoscopic examination. The incidence of Helicobacter pylori is highest between 41 50 years (72%) with male to female ratio of 2:3.Low socio economic status have a high incidence of Helicobacter pylori (65%) and (80%) in patients having a family history of dyspepsia. The colonization of helicobacter is related to life style i.e. habituation to smoking (76%), addicted to alcohol (36.5%) and beetle nut (3%). Higher incidence is in O+ve blood group. Non vegetarians are more for Helicobacter pylori infection than vegetarians and patients consuming spicy foods (80%) and pickles, chutneys containing excess chillies (90%). The biopsy specimens are collected in transport medium containing physiological saline and trimethoprim, processed under aseptic measures within two hours of collection. The standard tests, direct smear, rapid urease and culture were done. Helicobacter pylori was declared positive if the bacteria were identified by at least any two of the three diagnostic methods. Direct biopsy smear positive are 63, followed by rapid urease 53 and 49 are culture positive. Out of 49 culture positive cases, 31 are pure isolates of Helicobacter pylori and the rest are along with other organisms like Pseudomonas species, Proteus species and Klebsiella species. The colonisation is more common in pylorus (57.2%) than the anterior wall, posterior wall and duodenum. Forty nine isolates of Helicobacter pylori were subjected to antibiotic sensitivity testing. The incidence of Helicobacter pylori by various studies all over the world and in our country has been well documented and the present study is consistent with other studies all over the world. This study evaluates and is proved beyond doubt that there is a strong association between the presence of Helicobacter pylori and acid peptic diseases.
