Environmental perturbation such as pollution is one of the most disturbing aspects of environmental degradation. The Gulf of Gabes is presently facing severe environmental issues due mainly to intense fishing activity and improper industrialization (metal pollution). We have proposed in the present study, to assess the impact of environmental pollution on two fish species: Sardinella (S.) aurita and Mugil (M.) cephalus. To attend our objective, an exploration of morphological and asymmetry parameters was undertaken. The comparative analysis of the obtained data showed for S. aurita and M. cephalus, the existence of a significant difference between both male and female sexes, the total length (the standard length, the length to the fork and the predorsal length and the morphological variables total length, standard length, length into the fork, the caudal length of the peduncle, the height of fishes, dorsal fin length and anal fin length respectively for the two species. The study of asymmetry parameters revealed some asymmetries affecting the length of the head and the diameter of the eye, respectively for M. cephalus and S. aurita.