Object: In this present study easily available four cassia species like Cassia occidentalis, Cassia fistula, Cassia auriculata and Cassia alata were selected and they were qualitatively and quantitatively evaluated for their anthroquinone glycosides like Sennoside A Sennoside B and compared with Cassia angustifolia. The presence of these phytoconstituents were confirmed by HPTLC technique. The evaluation of traditional drugs is primarily based on Pharmacognostical, Phytochemical, Pharmacological and allied approaches including various instrumental techniques such chromatography, microscopy, spectroscopy and others. Senna is commonly found medication traditionally used by Arabian physicians primarily as a Cathartic. The laxative effect exerted by Senna is mainly attributed to anthraquinones which include dianthrone glycosides, sennosides A and B, sennosides C and D. The genus Cassia in the family Leguminosae in the major group angiosperm. Anthraquinones are functionally diverse aromatic chemicals, structurally related to anthracene, with parent structure 9, 10-dioxoanthracene. Results: The HPTLC spectrum shows the same Rf value in 4 species (Cassia fistula, Cassia occidentalis, Cassia auriculata, Cassia alata) and in Cassia angustifolia Rf value 0.45, 0.44, 0.46, 0.45, and 0.46 and Rf value 0.77, 0.76, 0.78, 0.79 and 0.79 respectively. Here, it was clearly verified the presence of Sennosides.
