Pseudomonas aeruginosa (P.A) is one of the major opportunistic pathogens of human often associated with morbidity and mortality. This study is to determine antibiotic susceptibility pattern and biofilm production among the P.A isolates and also compare biofilm production among carbapenem resistant clinical and environmental isolates from the selected Hospitals. Strains were recovered from different clinical and environmental samples from the hospitals using standard microbiological techniques. Kirby-Bauer disc diffusion method was used for susceptibility testing and biofilm production was determined using quantitative method. A total of 132 stains (120 clinical and 12 environmental isolates) of non duplicate P.A were recovered from 1,214 clinical and 1,000 environmental samples of the selected hospitals. Imipenem was the most effective antibiotics against the isolates as 84.2% (101) and 100% (12) were recorded from clinical and environmental isolates respectively. Biofilm production was 41.7% and 16.7% among clinical and environmental P.A and carbapenem resistant clinical P.A produces more biofilm than carbapenem resistant environmental P.A with 63.9% and 40% respectively. In conclusion policy maker and health practitioners should address this problem and implement a more rational and appropriate use of antibiotics. This may involve restricting and monitoring the sales of antibiotics in the country.
