Penaeussemisulcatus (grooved tiger prawn or green tiger prawn)accessions were collected from southern subcontinent of India and the anti-oxidant enzyme levels in their tissues were compared. The accessions were from Pondicherry, Pazhaverkadu, Royapuram and Thalankuppam. From the frozen tissue samples of these species, Lipid peroxidation, Catalase activity, Glutathione S Transferase, Reduced glutathione, Acetyl cholinesterase, and Superoxide dismutase enzyme levels were measured. All the enzyme levels were elevated in the species collected from Thalankuppam compared to other three species. This is possibly due to the associated environmental factors and is an indirect measure of pollution levels.