Seedlings of pigeonpea (Cajanus cajan L. Mill.) grown on treated with different cadmium (Cd) concentrations representing 0, 0.02, 0.04 and 0.06 mM were used in three pigeonpea cultivars, LRG30, LRG41 and ICPL85063 on total nitrogen content, nitrate reductase, malate dehydrogenase and glycolate oxidase activities were studied. Results obtained show that the pigeonpea cultivar, LRG30 registered higher values of nitrogen content in response to Cd treatment. The nitrate reductase activity and glycolate oxidase were affected more in the pigeonpea cultivars LRG41 and ICPL85063 in response to Cd treatment. The malate dehydrogenase, exhibited lower values in the pigeonpea cultivars, LRG41 and ICPL85063 than in LRG30 in response to Cd treatments. The effects were more conspicuous under Cd treatment.