An efficient protocol has developed for induction, maturation and plantlet germination. In this study the leaf explant of C. paniculatus inoculated into Murashige and Skoog Medium supplemented with different concentrations and combination of 2,4-D, BAP and TDZ. Maximum percentage of callus induction was observed induction was 86.5% in 2,4-D and BAP (1.0+0.5mg/lt) under the influence of red light treatment and 83.3%, 81.6%, and 78% under the influence of white, blue and yellow light treatment respectively. Maximum percentage of somatic embryogenesis 92% was observed in MS medium fortified with 2, 4-D (1mg/lt) + BAP (0.5mg/lt) under the influence of red light treatment, 90%, 88% and 87% under the influence of blue, white and yellow light treatment respectively. Maximum average number of plantlets 38.0±3.4 was observed in MS medium fortified with 2, 4-D and BAP (1.0+0.5mg/lt) under the influence of red light treatment and 33.6±3.2, 31.3±4.1 and 27.6±0.5 under the influence of blue, yellow and white light treatment respectively. We report for the first time the use of higher wavelength for an efficient rapid propagation of this threatened medicinally important plant. This protocol will prove suitable in overcoming the demand and supply ratio of this plant for medicinal use. At the same time it will help in conserving C. paniculatus in its natural habitat.
