The paper deals with problem of water quality become more important than quantity in parts of Mandakini River Basin, Chitrakoot district, Uttar Pradesh. Based on the integrated studies, it has been observed that the lithology, geomorphology, lineaments, slope, effluents, sewage disposal, soil and land use/land cover was generated from Modis, Landsat ETM + data and Survey of India, (Nos.63 C/15 and 63 C/16; Figure 1.1) on 1:50,000 scale and integrated data with raster based Geographical Information System (GIS) to identify the groundwater quality of an area. Water quality is also affected due to human activities like improper agricultural practice, industrial wastes and municipal solid wastes. In context to the above issues an attempt has been made to study the geochemistry of groundwater available in Karwi Area, Mandakini River Basin, Chitrakoot district, Uttar Pradesh. The water samples have been analysed for anions and cation. The anomalies are highlighted by the concentration maps chart and the same has been interpreted. The final result represents the favourable quality of the groundwater in the study area is suitable for both domestic and agricultural activity as of now.