The present study was conducted in Parthenium hysterophorus infested fallowland in the University campus of J.P. University Chapra, Bihar in 2017. This study was aimed at determining the impact of Parthenium on soil chemical properties. The soil samples were collected from 0-15 cm and 15-30 cm depth of Parthenium infested and 0-15 cm soil depth of non-infested sites. The present study indicated that in Parthenium infested sites values for pH, Organic carbon, Organic matter, N, total soluble salt content, Zn, Mn and Fe were lower compared to non-infested site whereas values for K, P and Mn were higher in infested site. However the differences in soil chemical properties of Parthenium infested and non-infested sites were not statistically significantly different.