Studying the importance of ENVIRON-AC an analytical investigation was performed for the accuracy and acceptability of the product. Regarding the microbial status, it was found that the total bacteria were found 50±1.45×109 cfu/gm, where Lactobacillus sp. and Bacillus sp. were 27±1.45×109 and 24±1.45×109 cfu/gm respectively. Protease enzyme was found in highest amount (90.22 U/gm). The product also showed antimicrobial activity against Vibrio harveyi MTCC No. 7954. The amount of total ammonia, total nitrate and total nitrite in the pond water were 0.324±0.004, 0.020±0.006 and 0.016±0.0007 ppm respectively and which were below the safe level by the application of ENVIRON-AC. The oxygen level and pH range of the treated pond were 4.2-5.1 mg L-1and 6.4-8.9 respectively. Average body weight of the shrimp in treated pond was 33±1.92 gm, which is 37.5% higher than control pond. Vibrio sp. was found very less in no. (0.03×105 cfu ml-1) and survival rate was 91% in treated pond.
