In the present investigation, plant growth promoting bacteria such as Pseudomonas and Bacillus were isolated from the rhizosphere soil of rice (Oryza sativa L.). Bacillus is a group of gram positive, aerobic or anaerobic bacteria widely found in soil and water. Bacillus is a common free - living species found in all types of soil and environment. Pseudomonas is gram negative rod shaped and polar flagellated bacteria found in most of the environment. During the isolation studies about ten different Bacillus and ten different Pseudomonas isolates were isolated and characterized based on biochemical studies. The isolated Bacillus and Pseudomonas isolates were designated as PGPBB1-PGPBB10 and PGPBP1- PGPBP10 respectively. All the twenty isolates of Bacillus and Pseudomonas were studied and screened for the IAA and GA3 production. In the production of IAA and GA3 all the isolates of pseudomonas recorded much significant values compared with Bacillus isolates from the rhizosphere soil of rice.