The purpose of this study was to investigation influencing factors on job satisfaction of employees in Agricultural Research, Extension, and Education organization (AREEO). The statistical population includes all employees in 66 institutes and centers in AREEO in Iran (N= 4630). Among them 357 employees (n=357) were selected by stratified random sampling method and finally 245 questionnaires were returned and analyzed. Data collection in this research was done in two general sections. Section one was associated with review of the literature, theories and models in order to identify influencing factors on job satisfaction of employees. These factors included the nature of the job, management, payments, work conditions, operational performance, and improve working conditions. Section two was done in order to examine the hypotheses by using Structural Equation Modeling (SEMs). The Results of this section showed that operational performance and improve working conditions, had significant effects on job satisfaction of employees in AREEO.