Vignamungo is an important pulse crop of South India which belongs to the family Fabaceae. Vignamungo also commonly known asblackgram is cultivated in wider areas of Tamil Nadu. The present investigation has been carried out to assess the influence of chromium on seedling growth, biochemical constituents and antioxidant properties of Vignamungo. Blackgram seeds were germinated under various concentrations of chromium (5, 10, 25, 50, 75 and 100 mgl-1). One set was irrigated with distilled water served as control (0 mgl-1). Seed germination rate, seedling growth parameters were recorded on the 9th day after sowing of seeds. The biochemical constituents and antioxidant enzymes were analyzed on the same day. All the growth parameters, biochemical constituents and antioxidant enzyme activities of blackgram decreased in proportion to the concentration of metal applied to the seedlings.