Aim: This study was performed to unmask the hematological and biochemical alterations occurred in blood and serum of infected buffaloes, respectively. Material & Method: A total of 50 blood and serum samples were collected from infected as well 10 non-infected buffaloes during the ante-mortem examination. Animals were bled from the jugular vein into 10ml vacutainer tubes containing EDTA (Ethylene DiamineTetraacetic Acid) for the estimation of various haematological parameters and 10 ml blood collected in serum clot activator tube to study biochemical parameters. Result: The infected buffaloes showed a significant reduction in the mean Rbcs, Hb, PCV, and lymphocyte and increased TLC, neutrophil and eosinophil. The monocytes level decreased and basophils levels increased non-significantly in infected buffaloes. In the biochemical profile significantly reduced in the values of total serum protein, AST/ SGOT, ALT/ SGPT and calcium Conclusion: Based on the results we got during study it can be concluded that Fasciola spp. Infectioncauses heavy liver and muscle damage and cause heavy loss of blood from the body.
