This study was conducted to estimate the effects of estradiol benzoate injection to intact and castrated male rabbits on glucose, total protein, albumin, calcium, urea and creatinine concentrations. A total of Seventy two adult male rabbits were used in this study. The rabbits were randomly divided in to two groups (Thirty sex per group) .One group was used as intact group, the other group of rabbits was subjected to bilateral orchiectomy, and used as castrated group. Each group was further divided randomly to four sub-group(six for each sub-group).Three sub-groups of intact and castrated rabbits were treated once each alternative day with the intramuscular injections of estradiol benzoate (Estradol ® Animal health care Australia) at a doses of(40, 80 and 120 μgm/ rabbit), respectively for thirty days, whereas the fourth sub-group of each receive placebo and act as control group. After the end of experiment period 30 days, a blood samples were collected, and the serum samples were being harvested for analysis of glucose, total protein, albumin, calcium, urea and creatinine concentrations.The study found that glucose concentration was significantly (P<0.05) increase and decrease in intact male rabbits, significantly (P<0.05) increase in castrated male rabbits, total protein significantly (P<0.05) increase or not changed in intact male rabbits, and significantly (P<0.05) decrease in castrated male rabbits. Albumin and calcium were not change (P˃0.05) in intact and castrated male rabbits. Urea was not changed in intact male rabbits and in castrated male rabbits not changed or significantly (P<0.05) increased. Creatinine was not changed or significantly (P<0.05) decrease in intact and castrated male rabbits. It conclude that estradiol benzoate may induced increase or decrease in glucose and total protein, increase in urea or no change, decrease in creatinine or no change, while albumin and calcium were not affected.
