The present study was carried out to evaluate the anti-inflammatory property of the ethanolic extracts of the whole plants in vitro method and it was estimated by Human Red Blood Cell membrane (HRBC) stabilization method. This method showed significant anti- inflammatory property of the different concentrations tested. The ethanolic extract at a concentration of 200µg/ml showed potent activity on comparing with the standard drug diclofenac sodium. The extracts exhibited the % of protection in a dose dependent manner. The physicochemical characters of Abutilon indicum Linn. was studied and it was observed that moisture content (3.2 %), total ash (40 %), water soluble ash (26.56 %), acid insoluble ash (25 %), alcohol soluble extractive value (6.89 %), water soluble extractive value (38.68 %) and fluorescence analysis were determined. The phytochemical screening was also carried out to find various phyto constituents present in the Abutilon indicum Linn. The qualitative phytochemical analysis of whole plants reveals the presence of alkaloids, glycosides, saponins, triterpenes, carbohydrate, steroid, protein, phytosterol, flavonoid, phenol, fixed oil and fats.