We investigated the mycoflora of poultry litter in some selected farms to determine their mycological composition and hence their public health implication. The study was done in twenty five poultry farms in Jos, Plateau State of Nigeria. Five samples of litter were collected from each farm for mycological analysis. Samples were cultured on Sabouroud Dextrose Agar and incubated at 25oC for three weeks. Positive cultures were identified through standard methods. Results indicated that 80.0% of poultry farms in the locality had mycotic infestation. All the isolates obtained are agents of human myscoses. The most encountered genus was of the Trichophyton species (28.0%) followed by Aspergillus species (26.0%) and Mucor species (20.0%). The least however was of the Malassezia species (2.0%). Also, the most encountered species were those of Mucor (20.0%) followed by Penicilliun notatum (16.0%) and Aspergillus niger (12.0%) while Malassezia furfur, Malassezia gypsium, Microsporium audouinii and some species of Trichophyton were the least encountered with a prevalence of 2.0%. These findings therefore suggest that poultry litter provides a good ecology for fungi thereby posing a great public health threat to humans
