This study was conducted to investigate the prevalence of Trichomonas vaginalis infection amongst female students of Benue State University, Makurdi. A total of 138 swab samples were collected using sterile swab sticks which were examined microscopically. Out of 138 samples, 39(28.3%) were positive for the parasite. According to age groups, 26-30years had the highest infection rate (6(54.5%)). With respect to marital status, married students had a higher infection rate (6(66.7%)) than single students. Pit latrine users had (13(86.7%)) and sexually active students had 13(46.4%). There was no significant differences between prevalence in terms of location, level of education, sharing of underwears and age (P>0.05). On the other hand, type of toilet, complaint status and sexual activity and marital status were significant (P<0.05). Trichomonas vaginalis is prevalent in Benue State University and suggests that hygiene should be intensified. It is therefore recommended that students of Benue State University be enlightened on the existence of Trichomonas vaginalis since so as to help reduce its spread.