Wild mushrooms are vitally important non-timber forest products. However, changes in biodiversity and the effects of climate change are influencing their seasonal availability. Different types of substrate were formulated for this study, including three based on sawdust and one based on banana leaves. Before spawning, the culture bags were sterilised and then divided into three groups according to the type of oyster mushroom species (Pleurotus cornucopiae, Pleurotus ostreatus and Pleurotus citrinopileatus). After spawning, the culture bags were stored in a dark room until they were completely invaded. They were then stored in a fruiting room. The parameters assessed were: stipe height, number of clumps, number of runts, average number of plants per clump, cap diameter, carpophore mass and yield. The results of the statistical analyses showed that the substrates had an influence on changes in the various parameters considered. The study carried out on the evaluation of the production potential of various oyster mushroom species showed that Pleurotus cornucopiae was the species that recorded a high yield on sawdust-based substrates compared with the other oyster mushroom species.