The four fishes were selected for the study of protein includes Catla catla, Labeo rohita, Cirrihinus mrigala, Cyprinus carpio from the Nathsagar region, Tal. Paithan dist. Aurangabad, from the present estimation. The experimental fishes were collected in the live condition in the local fresh water sources brought to the laboratory. The fishes of similar size were selected. Lowry’s ‘C’ method is used to calculate the protein value in muscle sample of selected fishes. The principle of Lowry’s ‘C’ method is the blue color developed by the reduction of phosphomolybdotungstic compounds in the folin's reagent. All reagent’s by the amino acids the color developed by the burette reaction of the protein with the alkaline measured in the Lowry’s method from the Lowry’s C method for estimation of protein in muscle sample is incubate the following protein contain in selected fishes. 1. Catla catla is showed protein contain in muscle 28 cal/100mg 2. Labeo rohita is showed protein contain in muscle 23 cal/100mg 3. Cirrihinus mrigala is showed protein contain in muscle 25 cal/100mg 4. Cyprinus carpio is showed protein contain in muscle 21 cal/100m
