Water resources in India have reached a point of crisis due to unplanned urbanization and Industrialization. Advanced human activities over the recent past years are causing greater threats on these freshwater resources, resulting in changing their features. A study of Physico-chemical parameters of Anjneri pond water was carried out during February 2015 to January 2016. Physical characters like Temperature, transparency, total dissolved solid, Rainfall and Chemical Parameters like Dissolved oxygen, pH, free carbon dioxide, Hardness (Ca & Mg), Total Alkalinity, Chlorides, and Biochemical oxygen Demand were studied during the study. It is observed that all the selected physico-chemical parameters show seasonal variation and also fluctuated by anthropogenic activities.