Biochemical composition study helps to assess the nutritive value of fish from a particular region in different seasons. The biochemical composition in the muscles of goby fish, Parachaeturichthys ocellatus from the creeks of Mumbai was estimated from July 2010 to September 2011. The range of values obtained for proximal composition of adult P.ocellatus were: 78.05-81.51% of moisture, 13.47-15.65% of protein, 0.8-2.55% of Lipids, 0.06-0.16% of carbohydrates and 2.37-3.87% of ash. P.ocellatus was found to be low fat and high protein fish.. Seasonal variation was observed in male and female P.ocellatus during different months but there was no significant difference in the biochemical composition in terms of content of moisture, protein, lipid, carbohydrate and ash. Total caloric value calculated was 979.07cal/g in male and 995.44cal/g in females. The caloric content of the P.ocellatus was maximum during pre-spawning months and minimum in post spawning months. The fish was found to be nutritious throughout the year.
