A study was undertaken on induced mutagenesis with two varieties of Soybean, BSS-2 and RKS- 18. The mutagen used was Gamma rays (50,100,150,200 and 400 Gy). A difference was observed between the varieties BSS-2 and RKS-18 in the degree of tolerance to the mutagens. Germination and survival percentage in both the varieties was lower as compared to control. Reduction in germination percentage was associated with increase in dose of mutagen in both varieties BSS-2 and RKS-18. Germination percentage was higher in the variety BSS-2 as compared to RKS-18, while a higher survival percentage of the seedlings was recorded in RKS-18 which showed a higher genetic damage in the variety BSS-2. In variety BSS-2 at 100 Gy dose a higher survival percentage 94.6% was recorded as compared to lower dose 50Gy while in RKS- 18 the lower dose of 50 Gy (79.4) showed much higher survival percentage in comparison to higher dose of 100 and 150 Gy.