Lycopene is a carotenoid found in nature, it is being used for the treatment of various disorders like cardiovascular diseases, osteoporosis, bone health, male infertility, skin protection, and age related muscular degeneration prevention. It exhibits the highest antioxidant activity and singlet oxygen quenching ability of all dietary carotenoid. The red fleshed watermelon juice samples were analysed by UV-VIS spectrophotometer, FTIR spectroscopy and GC-MS. The most predominant volatiles lipid forms of hydrocarbons were identified through GC-MS. The major chemical constituents are identified as N,N’- Ethylenebis [2-[2- hydroxyphenylglycine, Cyclopropanebutanoic acid, n- Hexadecanoic acid, 10-Octadecenoic acid, oleic acid, Heptadecanoic acid, Hexadecanoic acid, 9-octadecenoic acid. The chemical compounds found in groups are Methyl branched fatty acid, ethylester based fatty acid, Glycine based amino acid, and Palmitic acid and oleic acid. The results showed that lycopene content of watermelon juice of approximately 13mg/Kg. The extract of lycopene was subjected to invitro antioxidant activity using three different methods such as DPPH, hydrogen peroxide radical and reducing power assay method. The lycopene exhibited DPPH activity with an ARP value of 0.0035, hydrogen peroxide scavenging activity with IC50 value of 10.636 µg/ml. The extracts had shown significant antioxidant activity against method with IC50 value of 22.70µg/ml.
