Onion is one of important commercial vegetable crops grown in worldwide. India is second most onion producer. In India Maharashtra is leading state. Present study was investigated of the isolation of fungal diseases in the field of onion. Onions are infected from many fungal diseases, such as, Basal rot, southern blight, purple blotch, White tip, Bot¬rytis Squamosa,downy mildew, Neck rot, leaf blotch and black stack ofrotetc. Basal rot and Southern blight is the major fungal Bulb diseases. A complete causes the number of onion fields every year by fungi. Diseases were collected from the different varieties likewise, Nashik red, Punafursungi, Panchganga, Bhima kiranand N-53 and different localityof Maharashtra likewise, Aurangabad, Beed, Jalna, Ahemdnagar, Nashik, Osmanabad and Pune districts. Fungi were isolates from the infected onions Bulbs of different pathogen as like, Fusarium oxysporum,Botrytis allii,and Sclerotium rolfsiietc. isolated fungi in Maharashtra for this study.