The Jackfruit is a forest species whose cultivation is totally unknown in Côte d'Ivoire. Its reproduction by seeds is not easy because the seeds are recalcitrant. Objective study is to develop a protocol for in vitro propagation of Jackfruit plants in Côte d’Ivoire in order to have quality and quantity plants. Terminal buds and microcuttings fragments from forest and garden nursery were aseptized and cultured. The impact of Calcium hypochlorite (Ca (OCl2)) (2.5 to 7%) on aseptization of explants and the effect of Benzylaminopurine (BAP) (0.1 to 2 mg/L) on budding, Gibberellic Acid (GA3) (0.1 to 1.5 mg/L) on shoot development and Indole-3-Butyric Acid (IBA) (0.5 to 2 mg/L) on rhizogenesis were studied. Results show that with 3.5% Ca (OCl2) for 10 minutes incubation, 80% of garden buds survived against 30% of forest bud. At, 2 mg/L BAP, 88% of bud sprout within 5 days of cultivation. with 1.5 mg/L of GA3, all shoots reached an average size of 2.5 cm in 30 days of cultivation. A 40% of shoots were rooted on 2 mg/L IBA. The different hormones used are conducive to the culture of this specie . Conditions thus defined in this work constitute an method for producing plantlets of Jackfruit a view to the propagation and popularization of this species in Côte d'Ivoire.
