Leaf of Balanites aegyptiaca (Linn.) plant have been used as traditional folk medicines especially in Africa and southern Asia in the form of juice to treat diarrhea, curing anthrax, dysentery, cuts and clean malignant wounds. So this study is intended to evaluate the antioxidant potential and phytochemical contents in Balanites aegyptiaca (Linn.) leaf ethanol extract with GC-MS analysis and qualitative method according to standard protocol. Antioxidant potential of ethanol extract was found to be significant with IC50 value 37.61 ± 0.64 g/mL using DPPH (2, 2-Diphenyl-1-picryl hydrazyl) free radical scavenging assay method. The presence of various bioactive phytochemical contents in Balanites aegyptiaca (Linn.) leaf ethanol extract like carbohydrate, protein, amino acids, glycoside, tannins, saponin, flavonoids and phenolic compounds were found to be positive. The GC-MS analysis was carried out for the identification of active compounds in the extract and reported 12 phytochemicals, which are responsible for antioxidant activity. This evaluation revealed the antioxidant potency of leaf extract and its content active compounds role in reducing of oxidative stress related complications.
