Chickpea (Cicer arietinum L.) is a rich source of nutrients such as carbohydrates, proteins, fats, mineral ions and vitamins. Chickpeas crop yield has been affected by root knot nematodes infestation which account for approximately 13% of crop loss. Four main Meloidogyne species, namely M. hapla, M. javanica, M. arenaria and M. incognita attack chickpeas. In Kenya, chickpeas are cultivated after the main crops. This study was conducted in a glass house to characterize and identify the root knot nematodes attacking chickpeas in Nakuru County. Characterization and identification were done using perineal patterns procedure on female root knot nematodes. Thirty samples were taken and all resulted onto uniform perineal patterns of M. javanica distinguished from other species by a distinct lateral ridge separating dorsal and ventral arch. It was therefore concluded that M. javanica is the main root knot species attacking chickpeas in Nakuru County.