The aim of this work is to study the chemical composition of a Lebanese Propolis sample. Extraction of the sample with Ethanol 70% was first applied to a greenish powered propolis. The Total Phenolic Content and the Total Flavonoid Content of the Crude Ethanol Extract obtained showed 252.34 mg GAE/g propolis and 140 mg QE/g propolis respectively. This extract was then used for a Liquid-Liquid Fractionation process, using three successive organic solvents. Three different fractions were obtained: Hexane Extract, Methylene Chloride Extract and Ethyl Acetate Extract. By comparison with eight analytical standards, two Phenolic acids and six Flavonoids were identified and quantified in those organic extracts using a Reversed Phase High Performance Liquid Chromatography system (RP-HPLC). The chromatograms showed the presence of Ferulic acid (9.10%), Chrysin (5.94%), Pinocembrin (2.08%), Quercetin (1.91%), Kaempferol (0.48%), Galangin (0.26%), Caffeic acid (0.05%), and Apigenin (0.02%).
