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Antifungal efficacy of alkaloids, flavonoids and steroids of different parts of Carissa carandas Linn

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Author: 
Dr. Meenakshi Fartyal and Padma Kumar
Abstract: 

Evaluation of the antifungal activity of extracts from different parts (root, stem & leaf) of Carissa carandas (Apocynaceae) Linn. was carried out. Different parts were collected, dried and extracted by using well established methods for alkaloids, flavonoids and steroids. Screening was done by using ‘Disc Diffusion Assay’ against Candida albicans (Yeast), Aspergillus flavus & Tricophyton mentagrophyte (fungi). Minimum inhibitory concentration, Minimum fungicidal concentration & Total activity were studied. Mean and Standard Deviation have also been calculated. C. albicans found to be the most susceptible organism while T. mentagrophyte found to be resistant organism for all the tested extracts. Alkaloid extract of stem showed best activity (IZ= 10 mm, AI= 0.43±0.02, MFC= 0.625 mg/ml, MIC= 0.312 mg/ml, TA= 65.6 ml/g) against C. albicans while free flavonoids (IZ= 11 mm, AI= 0.24±0.02, MFC= 0.312 mg/ml, MIC= 0.156 mg/ml, TA= 73.7 ml/g) & bound flavonoids (IZ= 10 mm, AI= 0.22±0.02, MFC= 0.625 mg/ml, MIC= 0.312 mg/ml, TA= 8 ml/g) of stem showed very good activities against A. flavus. The range of MFC & MIC was found to be 1.25-0.312 mg/ml & 0.625-0.156 mg/ml, respectively. Results reveal good antifungal potential of extracts. Hence, may be explored for formation of new antifungal drugs.

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