The present study deals with the characterization, digestive enzymatic productivity and antibacterial activity of intestinal bacteria of Rohu Labeo rohita. Pure colonies were isolated from the intestinal content of Rohu was characterized morphologically and biochemically using the Berge’s manual of Determinative bacteriology. The isolated and identified intestinal bacteria was Aeromonas sp., (R1), Pseudomonas sp., (R2), Bacillus sp., (R3), Enterobacter sp., (R4) and E.coli sp., (R5). The isolated intestinal bacteria were subjected for its efficacy to produce digestive enzymes like amylase, cellulase, lipase, and protease were studied using selective media. The intestinal bacteria of Enterobacter sp., (R4) was higher enzymatic productivity and other was lower productivity. The antibacterial activity of intestinal bacteria was higher zone of inhibition was Enterobacter sp., (R4) with compare to the commercial antibiotics of Gentamycin. Based on the results the Enterobacter sp., (R4) was higher enzymatic productivity and higher inhibition of antibacterial activity of intestinal bacteria in Rohu. The presence of this organism in the intestinal flora of the fish enhance the probiotic nature and helps in the nutritional benefits of fish.
