Release of textile azo dyes from industries to the environment is an issue of health concern while the use of bacteria and plants has proved to be the best choice for remediation. The present study reveals that the comparative study of biodegradation of azo dyes from nanoparticles synthesized by using plant (Tridax procumbens) and bacteria (B. subtilis). This study investigate an efficient eco-friendly and sustainable route of silver nanoparticles preparation from 1mM aqueous solution of silver nitrate using leaf extract of T. procumbens and supernatant of B. subtilis (extracellular).After 48 hrs of incubation at room temperature, the color of the solution intensified to brown indicating the formation of silver nanoparticles. The silver nanoparticles were further characterized by UV-Visible Spectrophotometer and Scanning Electron Microscope (SEM). The degradation of the dye congo red by AgNPs synthesized from T. procumbens and B.subtilis after 120 minutes of illumination were found 67.56% and 68.80 % respectively and for crystal violet, biodegradation were showed 17.54% and 20.33 % respectively. The degradation of Brilliant greenby AgNPs synthesized using T. procumbens and B.subtiliswere found to be48% and 62.16% respectively. The degradation of Malachite green by AgNPs synthesized using T. procumbenswas found to be 27.45% whereas by AgNPs synthesized using B. subtiliswas found to be 48.88%. Comparatively the degradation of azo dyes was highest by B. subtilis (35.48%) as compared toT. procumbens (28.36%). As biodegradation is microaerophilic or aerobic process completely detoxified all the selected textile azo dyes, further efforts should be made to implement such methods for large scale dye wastewater treatment technologies.
