An ethnomedicinal survey was conducted on Tiwa Tribe of Morigaon district of Assam, India during 2015 to 2017 in the villages of Borchila, Ga-khujuwa, Kahua-ati, Gokhai-khonda and Dalang-ghat of Morigaon district of Assam, India. During this survey, it was found that this community used 45 valuable plant species of 31 different families to cure various diseases and ailments. They use these plants to cure the diseases like skin disease, eye infection, diarrhoea, rheumatism, tooth problems, stomach ache, hair fall problems, fertility problems, cuts and wounds etc. The plant parts were applied as paste, boiled decoction, juice extract from the fresh plant parts, powder made from the dried plant parts etc. The study also revealed that family Verbenaceae and Lamiaceae were represented by 03 plant species; Araceae, Acanthaceae, Rutaceae, Amaranthaceae, Solanaceae, Apiaceae, Rubiaceae, Zingiberaceae, Cyperaceae and Polygonaceae were represented by 02 plant species each whereas the rest of the families were represented by 01 species each.