Reproduction in crustaceans is one of the energy demanding process. In crabs various aspects of reproduction like egg maturation, ovulation, spawning and incubation of eggs are completed within the intermoult stage. Unilateral eyestalk ablation was performed in freshwater female crabs Oziotelphusa senex senex. Eye stalk ablated crabs were maintained for a period of 25 days along with non eyestalk ablated control crabs. Hepatopancreas and ovarian lipid profiles after the completion of 25 days in control and experimental group of animals were analysed. When compared to the control animals, all lipid classes (cholesterol, phospholipid, triglycerides and free fatty acids) showed a marked decrease in the hepatopancreas and a significant increase in ovarian tissue.