Weeds are unwanted and undesirable plants, which interfere with the utilization of land and water resources and thus adversely affect human welfare. Most of these plants propagate vegetatively by stolon and sexually by seed germination process. The Pectin methylesterase (PME) plays important role in the seed germination process. The PME, acts on the homogalactouran pectin of the cell wall affecting its porosity and elasticity to induce the water uptake initiating the seed germination. In the previous works, we have characterized PME, form Arabidopsis thaliana and identified natural potent inhibitor as catechin by using in-silico approaches. In the, continue of previous work here we have, studied the effect of catechin isolated from the Green tea on the seed germination of selected weed plants.