Objectives: The aim of this study was to evaluate nurse’s knowledge of evidence-based practices (EBPs) regarding the prevention of Ventilator-associated pneumonia (VAP) in ICU. Background: Ventilator-associated pneumonia rates are regarded as the most important indicator of quality of care in hospitals. Methods: A cross-sectional survey was conducted in seven hospitals in Turkey. A questionnaire consisting of 9 closed questions was used following the VAP prevention guideline of Dodek at al. (2004). Results: Results of the study found the nurses’ knowledge of EBPs regarding the prevention of VAP was low. In addition, there were no significant differences in mean knowledge by sociodemographic variables except for age and gender. Conclusion: Knowledge levels were not affected by years in ICU, years in the profession, and ICU certification. Results of the study suggested ICU nurses should have a certificate and in service training. However, the clinical guidelines and care protocols for prevention of VAP have been updated and implemented as a standard care for patients on ventilators in the ICU.