Isolation And Identification Of Probiotic Strains From Danisco Premix Yogurt Culture

Probiotics are alive and nonpathogenic microorganisms that are beneficial to maintain host intestinal balance. It was isolated from YO-MIX, Yogurt Cultures 38360 Sasssenage, DANISCO FRANCE by using Trypticase, Phytone peptone yeast extract (TPY) agar added 0.5% propionoic acid as selective agent and inoculated at 37 oC for 48 hours in anaerobic condition. Test of the colonies showed Y and V shaped. The Biochemical test for indole, catalase, gelatin, nitrate reduction, gas from glucose and arginine showed negative for all samples. According to identify the LAB probiotic strains milk samples inoculated with MRS agar media and the colonies morphology were observed under the microscope and found Rod shaped. Based on the microbiological and biochemical analysis most of the colonies were probiotic mix culture i.e Y, V shaped bifidobacteria and Rod shaped Lactobacilus Strains.