The relationship between biochemical tests and myocardial infarction has been studied. The study performed in 75 patients (45 males, 30 females) that had chest pain. The myocardial infarction tissue (MI) occurred in the locale of the hypoperfused myocardium. The whole blood taken from patients (3ml) was suffering from chest pain. Furthermore, the blood was estimated by Nano-Check in cardiovascular marker cTnl, CK-MB, and myoglobin. The patients revealed that had normal tests in spite of having chest pain. These results were taken in emergency hospital indicated that eleven patients hadpositive results, but other had negative results.