Sequel to the use of the plant parts of P. santalinodes (Nturu Ukpa) in traditional medicine the phytochemical and antimicrobial analysis of the leaves and stem were undertaken. Qualitative and quantitative evaluation of the phytochemical was made using standards methods. The microbiological analysis of the ethanol and methanol extract of the samples were performed on some test organisms Escherichia. coli, Staphylococcus. aureus, Klebsiella. pneumonia, Pseudomonas. aeruginosa, Bacillus. subtilis, Aspergillus. niger, Aspergillus. flavus, Trichophyton. rubrum and Microsporium. gypseum, using agar diffusion method for the screening, nutrient agar and potatoe dextrose agar were used respectively for the invitro antimicrobial activities. The results shows that, the plant part is rich in bioactive substances such as alkaloid (2.64±0.01), flavonoid (2.0±0.00), tannin (1.52±0.01), Saponnin (2.5±0.02), terpens (2.6±0.01), cardiac glycoside (2.5±0.00) and Steroid (1.82±0.01). The antimicrobial analysis shows that ethanol extract of the leaves had more inhibition against S.aureus,E.coli,B. subtilis, K. pneumonia and P. aeruginosa, than the methanol extract of the stem bark. The antifungal activities of the leaves and the stem bark showed a higher inhibition on A.niger, A.flavus, T.rubrum and M.gypseum on 40mg/ml concentration of the extract. The minimum inhibitory concentration (MIC) evaluated on the ethanol and methanol extract using a two-fold serial dilution showed little zone of inhibition ranging from (1-3mm) in diameter. The anti nutrient composition presents are in negligible amount. These results indicate that the plant leaves and the stem bark have potential medicinal uses, and could protect the body against some common microorganisms.
