Rapid micropropagation of md2 pineapple (ananas comosus l.) Using the temporary immersion system (tis) and liquid-shake culture (lsc)

Efficient and rapid vegetative micro propagation of MD2 pineapple was achieved using two liquid culture medium systems, viz. a culture vessel system (Temporary Immersion System, TIS) and liquid-shake culture (LSC). Multiplication of plantlets using these systems was better than on solid agar medium. Compared with the solid agar medium, the number of plantlets obtained by the liquid media systems was significantly higher, attaining an increase of up to four-fold. These in vitro systems are easy to manage and they facilitate uniform production of high quality MD2 pineapple plantlets. They are also amenable to up-scaling for commercial production.