The present investigations were undertaken on laboratory studies of Trichogramma japonicum (Ashmead) during the year 2013-2014 in the Bio-control laboratory, Department of Agricultural Entomology, College of Agriculture, Dapoli (Maharashtra).The results of effect of different insecticides on parasitisation potential of T. japonicum revealed that insecticides viz., oxydemeton methyl and cypermethrin could be used safely before T. japonicum release in the field. Further, insecticides viz., dimethoate, indoxacarb and emamectin benzoate could be used before T. japonicum release in the field with some safe period, while insecticides viz., malathi on and dichlorvos should not be used in the field, as they can disturb the parasitisation.