A study has been carried out for the evaluation of water quality Index in Dudhani village. Open well water sample were taken from Chokipada, Ruepada, Tokarpada, Kalmadevi,Navapada and Boripada locations and observed for the chemical and biological parameters of pH, Color, TDS, Turbidity, Total Hardness, Total Alkalinity, Chloride, Sulphate, Nitrate, Calcium, Magnesium, Iron, Zinc, Fluoride, E.Coli, and Total coliform bacteria. Iron value was observed high in all the sampling locations. Biological test parameters found positive in location 1,2,3,4,5 and 6. As per the WQI Classification of water, Very poor water quality was Identified in all eight sampling location. Test results of Chemical and Biological parameters indicate the water purification requirement to enhance the water quality for drinking purpose.